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Nocodazole pipette to depolymerize microtubules at the 8-cell stage.

Organism : Ascidian
Species : Phallusia mammillata
Description : Embryos at the 8-cell stage (interphase) were bathed in Cell Mask orange to label the mitotic spindle poles and the plasma membrane and during mitosis a nocodazole pipette was applied to the surface of one B4.1 blastomere. Note the exaggerated movement of the spindle pole nearest the nocodazole pipette towards the surface of the blastomere and CAB. Time in min:sec.
Attributions : Costache Vlad, McDougall Alex
Publication : Kif2 microtubule depolymerase is required for unequal cell division and localizes to a subdomain of cortical endoplasmic reticulum
Website : http://biodev.obs-vlfr.fr/fr/equipes_de_recherche/fecondation_et_controle_du_cycle_meiotique.html
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